朱兆鸿 黄开国 高白荷 李宗惠

云南省疟疾防治研究所  思茅 665000

全文发表于《实用寄生虫病杂志》1994年2卷2期30-31页

 

提要：本文报告用蜡烛缸平皿法培养恶性原虫配子体，对云南分离的FCCBH₁／YN和FccMD₁两株进行培养获得成功，两株分别在4d和1d出现配子体，16d和10d达高峰。最高密度分别为135和123/10000RBC，V期配子体最高密度为20和30/10000RBC，平均8.3和4.3/10000RBC。雌雄配子体比例为9:1和5:1，雄配子体可见出丝1－6条，表明配子体已达到成熟阶段，为国内首次报道。

关键词：恶性疟原虫，配子体，体外培养

 

GAMETOGENESIS IN CONTINUOUS CULTURE OF PLASMODIUM FALCIPARUM

Zhu Zhaohong, Huang Kaiguo, Gao Baihe, Li Zonghui

Institute of Malaria prevention and Treatment of Yunnan Province, Simao  665000

 

ABSTRACT：Continuous culture of two isolates of Plasmodium falciparum collected from two malaria patients in Yunnan was carried out with the established candle jar method. Young gametocyte first appeared on day 4 or 1 and the gametocytes peaked on day 16 or 10.The peak gametocytes parasitemina reached 135 and 123/10000 RBC respectively for two isolates. The highest density of matured gametocytes was 20 and 13/10000 RBC and the ratio between macrogamete and microgamete was9:1－5:1. Rounded microgametes with 1-6 flagella were clearly seen indicating gametocytes attained to sexual maturity. But experimental infection of An.dirus and An.minimus with the viable cultured parasites was unsuccessful.

Key words：Plasmodium falciparum,gametocyte, in vitro cultivation

 

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