余文祥  李菊升  李崇珍  余  蕾  黄云芝     云南省疟疾防治研究所（思茅665000）

全文发表于《中国寄生虫防治杂志》1993年第6卷第3期161-162页

 

提要：用间日疟病人血，离体感染中华按蚊，分离子孢子，用10～12万条子孢子／次的量，免疫BALB／c小鼠，抗体效价均达到1:640，取其脾细胞作融合试验，已培养出抗体滴度高、特异性强的抗间日疟原虫子孢子单克隆抗体（McAb）。

关键词：间日疟原虫子孢子，免疫

 

STUDIES ON IMMUNIZATION OF BALB/c MICE WITH PLASMODIUM VIVAX SPOROZOITES

Yu Wen-Xiang, Li Ju-Sheng, Li Chong-Zhen, Yu Lei, Huang Yun-Zhi

Institute of Malaria Prevention and Treatment of Yunnan Province  Simao  665000

 

ABSTRACT. The blood taken from Plasmodium vivax patients was used for memebrane-feeding An. Sinensis wiedmanns. 15 day later or so, the sporozites were separated. Each mouse was immunized with100-200 thousand sporozoites per time according to a certain schedual. All the titers of antibody to sporozoites from immunized mice reached to 1:640. The spleen cells from immunized mice were fused with SP2/0 cells to obtain the monoclonal antibodies to P. vivax sporozoites with high titers and specificity.

Key words:  P.vivax sporozoite，immunization，mouse，monoclonal antibody

 

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